Download pdf
Culture optimization and regulation mechanism of sporosarcina pasteurii for restoring root splitting fissures in Jinyang ancient city
- 1 Taiyuan University of Technology
- 2 Taiyuan University of Technology
- 3 Taiyuan University of Technology
- 4 Taiyuan Institute of cultural relics and Archaeology
* Author to whom correspondence should be addressed.
Abstract
To master the culture optimization and regulation mechanism of Sporosarcina Pasteurii, this study explored the adaptability of MICP technology for restoring root splitting fissures of plants, and used single factor comparative experimental method to study the characteristics of growth curves and urease activity of Pasteurii in different conditions in earthen sites of Jinyang Ancient City. The conclusions are as follows. The technology of MICP was an ideal method for restoring root splitting fissures of plants, which could meet the requirements of restoring earthen sites by sealing surface fissures, inhibiting root growth of plants and preserving the physical morphology of damaged plant roots and stems. It was recommended to use the medium of M1, with an initial pH of 7.25, a culture temperature of 30 ℃, inoculation age range of 12-13h, and inoculation amount of 3%. The suggestion was to select Pasteurii as restoration solution when the urease activity was at peak period. The conclusions can provide suitable breeding conditions for Pasteurii, providing an important reference for MICP technology to restore root splitting fissures of plants for earthen sites.
Keywords
Jinyang Ancient City, root splitting fissure, restoration, Sporosarcina Pasteurii
[1]. Liu X B, Meng H, Wang Y L, Katayama Y and Gu J D 2018. Water is a critical factor in evaluating and assessing microbial colonization and destruction of Angkor sandstone monuments J. Int. Biodeterior. Biodegrad., 133，9-16.
[2]. Wang R X 2009. Study on biomineralization process of calcium carbonate mediated by microbe and its application in restoration of cement-based materials defects
[3]. Whiftin, V S, vail Paassert L and Harkes M P 2007. Microbial carbonate precipitation as a soil improvement technique J. Geomicrobiol. J., 24,417-23.
[4]. Boquet'E，Boronat A and Ramos-Cormenzana A 1973．Production of calcite(calcium carbonate) crystals by soil bacteria is ageneral phenomenon J．Nature，246(5434),527-9．
[5]. Adolphe J P and Billy C 1974. Biosynth6se de calcite par line association bact6rienne a&obieJ. C. R. Acad., 278, 2873-75.
[6]. Adolphe J P , Hourimeche A,Loubiere J, Paradas J and Soleilhavoup F 1989. Les formations carbonatees d'origine bacterienne; formations continentales d'Afrique du Nord,BSGF-EARTH SCI B,55-62
[7]. Castanier S G,Le Metayer-Levrel and Perthuisot J P 1999.Ca-carbonates precipitation and limestone genesis-the microbiolgeologist point of view J．Sediment. Geol.，126，9-23
[8]. Wu S F,Zhang Y，Su M,He D P,Li J and Feng H Y 2022.Advancement of biotechnology for the conservation and restoration of cultural heritage objects，Sciences of Conservation and Archaeology，1，133-43
[9]. Liu X B, Meng H, Wang Y L, Katayama Y and Gu J D 2018. Water is a critical factor in evaluating and assessing microbial colonization and destruction of Angkor sandstone monuments J. Int. Biodeterior. Biodegrad., 133，9-16.
[10]. Adolphe J P，Loubiere J F，Paradas J and Soleilhavoup F 1990．Procédé de traitement biologique d'une surface artificielle P.European patent 90400G97
[11]. Wei H 2023.Experimental study and mechanism analysis of microbial mineralization in repairing high-temperature concrete damage
[12]. Fan Y N 2022.Study on mechanism and multi-scale performance of biomineralization for repairing fire damage of concrete
[13]. Xu Y B,Qian C X and Lu Z W 2013.Study on enhancing urease thermostability of Bacillus pasteurii by glycerol J.Journal of Southeast University (Natural Science Edition),43(1),147-51
[14]. Qian C X, Wang J Y, Wang R X and Cheng L 2009.Corrosion protection of cement-based building materials by surface deposition of CaCO3 by Bacillus pasteurii,Materials Science and Engineering: C,29(4),1273-80
[15]. Sun X H 2019. Study on mechanism of microbial mineralization and application of it to repair concrete cracks
[16]. Wei H, Fan Y, Sun L, Du H and Liang R 2022. Experimental Study on High-Temperature Damage Repair of Concrete by Soybean Urease Induced Carbonate Precipitation J. Materials, 15(7):,2436.
[17]. Xiao L 2004 Experimental techniques for environmental microorganisms (Beijing:China Environmental Science Press)
[18]. Shen P 2006 Microbiology (Beijing: Higher Education Press)
Cite this article
Shang,R.;Du,X.;Han,P.;Liu,W. (2024). Culture optimization and regulation mechanism of sporosarcina pasteurii for restoring root splitting fissures in Jinyang ancient city. Advances in Engineering Innovation,5,33-43.
Data availability
The datasets used and/or analyzed during the current study will be available from the authors upon reasonable request.
Disclaimer/Publisher's Note
The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of EWA Publishing and/or the editor(s). EWA Publishing and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content.
About volume
Journal：Advances in Engineering Innovation
© 2024 by the author(s). Licensee EWA Publishing, Oxford, UK. This article is an open access article distributed under the terms and
conditions of the Creative Commons Attribution (CC BY) license. Authors who
publish this series agree to the following terms:
1. Authors retain copyright and grant the series right of first publication with the work simultaneously licensed under a Creative Commons
Attribution License that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this
series.
2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the series's published
version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial
publication in this series.
3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and
during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See
Open access policy for details).