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Principles and applications of fluorescent probe imaging technology
- 1 Chongqing Weiming School
* Author to whom correspondence should be addressed.
Abstract
Fluorescent probe technology was discovered in the 19th century and imaging techniques were applied to microscopy around 1670. Fluorescence microscopic imaging is a classical method for observing the structure of organisms in the life sciences. In this regard, single-molecule fluorescence imaging uses fluorescent probes to label, detect, and analyze individual molecules, helping scientists to clearly observe the activities of individual molecules without disrupting the normal physiological state of the organism. In this paper, the principles and applications of fluorescent probe imaging techniques are described and analyzed. The common detection methods of fluorescent probes are spectrophotometry, electrochemistry, atomic absorption spectroscopy, high performance liquid chromatography, and fluorescence spectroscopy. Rapid detection results can be obtained depending on the specific method.
Keywords
fluorescent probe technique, imaging, microscopy, fluorescent protein, biology field
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Cite this article
Li,J. (2023). Principles and applications of fluorescent probe imaging technology. Theoretical and Natural Science,6,39-44.
Data availability
The datasets used and/or analyzed during the current study will be available from the authors upon reasonable request.
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Volume title: Proceedings of the International Conference on Modern Medicine and Global Health (ICMMGH 2023)
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