
Gibberellic Acid and Abscisic Acid Effects on Germination Across Arabidopsis Genotypes: Confirmation of Compound Identity and Genotypic Responses
- 1 St. Michael's College, University of Toronto, Toronto, Canada.
* Author to whom correspondence should be addressed.
Abstract
It is well known that plant growth depends on the interaction of auxin and hormones, but whether different hormones have different effects on seed growth in different varieties is not fully understood. This study used a controlled experimental setup in which seeds from three different genotypes (assumed to be wild-type, abscisic acid (ABA) insensitive, and gibberellic acid (GA) deficient) were exposed to treatments of these compounds (labeled compounds A and B), as well as a control treatment using ethanol. Our results confirm the identities of Compound A as gibberellic acid and Compound B as abscisic acid through their consistent effects on seed germination. Gibberellic acid (Compound A) significantly enhanced germination across all genotypes. With the help of Gibberellic acid, GA-deficient genotype also showed a dramatic increase in germination rates which suggested a compensatory effect. Conversely, abscisic acid (Compound B) markedly inhibited germination, with the most pronounced effect observed in the wild-type genotype. The ABA-insensitive genotype demonstrated reduced susceptibility to Compound B, supporting its phenotypic characterization. The study highlights the key antagonistic role of gibberellic acid and abscisic acid in seed germination, providing valuable insights into defining genotypic-phenotypic interactions in plant responses to environmental and chemical signals.
Keywords
Gibberellic Acid, Abscisic Acid, Germination, Plant Development
[1]. Mukherjee, A.; Gauray, A.K.; Singh, S.;Yadv, S.; Bhowmick, S.; Abeysinghe, S.; Verme, J.P. (2022). The bioactive potential of phytohormones: A review. Biotechnol Rep (Amst) 35, e00748.
[2]. Koornneef, M., & Meinke, D. (2010). The development of Arabidopsis as a model plant. The Plant journal : for cell and molecular biology, 61(6), 909–921. https://doi.org/10.1111/j.1365-313X.2009.04086.x.
[3]. Liu,X.; Hou,XL. (2018). Antagonistic Regulation of ABA and GA in Metabolism and Signaling Pathways. Front Plant Sci 9,251.
[4]. Shu, K.; Zhou, W.; Chen, F.; Luo, X.; Yang, W. (2018). Abscisic Acid and Gibberellins Antagonistically Mediate Plant Development and Abiotic Stress Responses. Front Plant Sci 9, 416.
[5]. Weiss, D.; Ori, N.(2007). Mechanisms of Cross Talk between Gibberellin and Other Hormones. Plant Physiol, 144(3):1240-1246.
[6]. Gupta,R.; Chakrabarty, S.K.;(2013). Gibberellic acid in plant. Plant Signal Behav 8(9):e25504.
[7]. Finkelstein, R. Abscisic Acid Synthesis and Response. Arabidopsis Book 11:e0166.
[8]. Geshnizjani, N.; Snoek,B.; Willems, L.; Bienstra, J.; Nijveen, H.; Hilhorst, H.; Ligterink, W. (2022). Detection of QTLs for genotye * environment interactions in tomato seeds and seedlings. Plant Cell Environ, 43(8):1973-1988.
[9]. Zhao, H.; Zhang, Y.; Zheng, Y.; Integration of ABA, GA, and light signaling in seed germination through the regulation of ABI5. (2022). Front Plant Sci. 13:1000803.
[10]. Castaño G.; Cabrera.C.; Pernas, M.; Gómez, L.; Sánchez,L.; An updated overview on the Regulation of Seed Germination. (2020). Plants. 9(6):703.
[11]. Finkelstein, R.R.; Gampala, S.S.L.; Rock, C.D. (2002). Abscisic Acid Signaling in Seeds and Seedlings. Plant Cell 14, s15-s45).
[12]. Finkelstein, R.R.; Somerville, C.R.(1990). Three classes of Abscisic Acid – Insensitive Mutations of Arabidopsis Define Genes that Control Overlapping Subsets of ABA Response. Plant Physiol, 94(3):1172-1179.
[13]. Peng,J.; Harberd, N.P. (1997). Gibberellin deficiency and response mutations suppress the stem elongation phenotype of phytochrome-deficient mutants of Arabidopsis. Plant Physiol 113(4);1151-1058.
Cite this article
Xu,X. (2024). Gibberellic Acid and Abscisic Acid Effects on Germination Across Arabidopsis Genotypes: Confirmation of Compound Identity and Genotypic Responses. Theoretical and Natural Science,54,55-62.
Data availability
The datasets used and/or analyzed during the current study will be available from the authors upon reasonable request.
Disclaimer/Publisher's Note
The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of EWA Publishing and/or the editor(s). EWA Publishing and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content.
About volume
Volume title: Proceedings of ICBioMed 2024 Workshop: Workshop on Intelligent Medical Data Analysis for Precision Medicine
© 2024 by the author(s). Licensee EWA Publishing, Oxford, UK. This article is an open access article distributed under the terms and
conditions of the Creative Commons Attribution (CC BY) license. Authors who
publish this series agree to the following terms:
1. Authors retain copyright and grant the series right of first publication with the work simultaneously licensed under a Creative Commons
Attribution License that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this
series.
2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the series's published
version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial
publication in this series.
3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and
during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See
Open access policy for details).