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Development and Application of Transcript Sequencing
- 1 Department of School of Public Health, Xiamen University, Xiamen, China
* Author to whom correspondence should be addressed.
Abstract
Transcriptome sequencing has become one of the mainstream methods for detecting gene expression after the development of high-throughput sequencing technology. We present the trajectory of the technological evolution of sequencing technologies between three generations and discuss their technical implementations, advantages, and disadvantages. Next, we show examples of this technology through the current mainstream NGS technology-based RNA-seq sequencing and analysis. We also look forward to the progress of the third-generation sequencing technology in read length and variable splicing detection.
Keywords
RNA-seq, sequencing technology, Nanopore
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Cite this article
Zhang,H. (2025). Development and Application of Transcript Sequencing. Theoretical and Natural Science,89,1-6.
Data availability
The datasets used and/or analyzed during the current study will be available from the authors upon reasonable request.
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Volume title: Proceedings of the 3rd International Conference on Modern Medicine and Global Health
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