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Published on 13 March 2025
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Fu,Z. (2025). Exploring the application of Bacillus Subtilis Spore Surface Display System in the Field of Catechol Degradation. Theoretical and Natural Science,99,1-15.
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Exploring the application of Bacillus Subtilis Spore Surface Display System in the Field of Catechol Degradation

Zi Fu *,1,
  • 1 Shanghai Pinghe Bilingual School, Huangyang Road, Shanghai, China

* Author to whom correspondence should be addressed.

https://doi.org/10.54254/2753-8818/2025.21329

Abstract

Catechol is a pollutant commonly found in industrial and everyday wastewater, with genotoxic effects that can lead to mutations, DNA fragmentation, and chromosomal aberrations. Traditional physical and chemical degradation methods face challenges such as high costs and secondary pollution, whereas biological degradation offers a cost-effective and environmentally friendly alternative. This study investigates the use of the Bacillus subtilis spore surface display system for catechol degradation, aiming to develop an efficient, low-cost biodegradation method. Recombinant Bacillus subtilis was successfully engineered to display catechol 1,2-dioxygenase (C12O) and catechol 2,3-dioxygenase (C23O) on the spore surface. Immunoblotting and immunofluorescence confirmed protein display and enzyme activity assays demonstrated catechol degradation activity. The displayed enzymes showed stability across a wide pH and temperature range, maintaining activity even under methanol conditions. Compared to purified proteins, the spore-bound enzymes eliminated purification steps, reducing costs and improving degradation efficiency in some conditions. These results suggest that the Bacillus subtilis spore display system has great potential in biocatalysis, offering a novel strategy for environmental pollution remediation.

Keywords

Catechol degradation, spore surface display, catechol dioxygenase

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Cite this article

Fu,Z. (2025). Exploring the application of Bacillus Subtilis Spore Surface Display System in the Field of Catechol Degradation. Theoretical and Natural Science,99,1-15.

Data availability

The datasets used and/or analyzed during the current study will be available from the authors upon reasonable request.

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About volume

Volume title: Proceedings of the 5th International Conference on Biological Engineering and Medical Science

Conference website: https://www.icbiomed.org/
ISBN:978-1-80590-007-8(Print) / 978-1-80590-008-5(Online)
Conference date: 17 October 2025
Editor:
Series: Theoretical and Natural Science
Volume number: Vol.99
ISSN:2753-8818(Print) / 2753-8826(Online)

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