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Optimization and Practical Evaluation of Multiplex PCR Technology for Toxin Detection in Food Science
- 1 The College of Food Science and Biomedical Engineering, Henan University of Animal Husbandry and Economy, Zhengzhou, Henan, China, 450000
* Author to whom correspondence should be addressed.
Abstract
The optimization of multiplex PCR technology involves factors such as primer specificity, annealing temperature, and buffer concentration. This paper discusses the optimization process of the technology and its performance evaluation in practical detection. The objective of the study is to enhance the sensitivity, specificity, and stability of multiplex PCR technology to more efficiently detect harmful substances in food. The research systematically evaluates the performance of the optimized PCR system by optimizing parameters such as primer design, annealing temperature, and buffer concentration. The sensitivity and specificity of the optimized technique are validated through experimental data, demonstrating advantages in stability and reproducibility. In practical applications, the optimized technology was tested on food samples, providing specific data analysis and comparing its performance with existing detection methods. A comprehensive evaluation of multiplex PCR's application value in terms of detection time, cost, and efficiency was conducted. The results show that the optimized multiplex PCR technology improves the detection sensitivity to 0.1 pg/µL, with specificity exceeding 98%, along with lower background signals and higher stability. The widespread application of this technology is expected to provide a faster, more accurate, and cost-effective detection method for food safety testing.
Keywords
Multiplex PCR, Food Safety, Harmful Substance Detection, Technology Optimization
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Cite this article
Sun,H. (2025). Optimization and Practical Evaluation of Multiplex PCR Technology for Toxin Detection in Food Science. Theoretical and Natural Science,94,63-66.
Data availability
The datasets used and/or analyzed during the current study will be available from the authors upon reasonable request.
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Volume title: Proceedings of the 3rd International Conference on Environmental Geoscience and Earth Ecology
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