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ATAC-seq: A powerful tool for investigating chromatin accessibility and transcription factor binding
- 1 Shenyang Agricultural University
* Author to whom correspondence should be addressed.
Abstract
Chromatin Transposase Accessibility Sequencing is a new high-throughput sequencing technique developed by Professor William Greenleaf in 2013 which uses DNA transposase to probe chromatin accessibility with Tn5 transposase. This technique, which is simpler and more sensitive than DNase-seq, MNase-seq and FAIRE-seq and requires fewer cells, has been used to study chromatin accessibility using Tn5 transposase. ATAC-seq is important for the study of epigenetic molecular mechanisms because it can map chromatin accessibility on a genome-wide scale, compare open chromatin regions in different tumour samples, compare differences in transcription factor binding between treatments, reveal nucleosome localisation information and transcription factor binding sites, and can be used to locate specific unknown transcription factors, which can be used in combination with other methods to screen for specific transcription factors of interest. It is possible to combine this approach with others to investigate specific regulatory factors. Herein, ATAC-seq is systemically profiled to present that ATAC-seq has enormous potential to drive future discoveries in the field of genomics and beyond.
Keywords
ATAC-seq, Tn5 transposase, chromatin accessibility, epigenetic molecular mechanisms, genome-wide scale
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Cite this article
Wang,X. (2023). ATAC-seq: A powerful tool for investigating chromatin accessibility and transcription factor binding. Theoretical and Natural Science,6,316-322.
Data availability
The datasets used and/or analyzed during the current study will be available from the authors upon reasonable request.
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