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CRISPR/Cas system: A powerful tool for de-extinction
- 1 Guangdong experimental high school
* Author to whom correspondence should be addressed.
Abstract
The rapid advances in CRISPR technology have opened up new avenues of research and provided hope for a future where extinct species can be brought back to life. De-extinction, the process of resurrecting extinct species by using genetic engineering techniques, is one potential application of CRISPR technology that has gained increasing attention in recent years. The idea is to recover the genetic information of extinct species from preserved tissue samples and recreate them using modern genetic engineering techniques. George Church, a geneticist at Harvard University, is one of the leading scientists exploring the potential of CRISPR for de-extinction. He has spearheaded efforts to resurrect the woolly mammoth, which went extinct over 4,000 years ago, by inserting mammoth DNA into the genome of the Asian elephant. While this project is still in its early stages, it has sparked renewed interest in using CRISPR to bring back other extinct species, such as the Pyrenean ibex, the passenger pigeon, and the Tasmanian tiger. This review paper aims to explore the potential of CRISP technology for de-extinction, including its technical and ethical challenges, and the progress that has been made in various de-extinction projects involving CRISPR technology.
Keywords
De-extinction, CRISPR/Cas system, genetic engineering
[1]. Siewert M B, Hanisch J, Weiss N, et al. Comparing carbon storage of Siberian tundra and taiga permafrost ecosystems at very high spatial resolution[J]. Journal of Geophysical Research: Biogeosciences, 2015, 120(10): 1973-1994.
[2]. Ying YAN,Roswitha A. AUMANN,Irina H?CKER,Marc F. SCHETELIG.CRISPR-based genetic control strategies for insect pests[J].Journal of Integrative Agriculture,2023,22(03):651-668.
[3]. XUE Shan,WANG Shuya,LIU Li,ZHONG Qiaofang,CHENG Zaiquan,XIAO Suqin. Research progress of precision gene editing technology based on CRISPR/Cas9[J/OL]. Chinese Journal of Biotechnology:1-13[2023-04-16].
[4]. Huang Meiyan, Liu Binghuan, Jie Yonghong, Zhao Hong, Shang Chenyang. Off-target effects and countermeasures of CRISPR/Cas9 system [J/OL]. Molecular Plant Breeding: 1-19 [2023-04-16].
[5]. Ryu S M, Hur J W, Kim K. Evolution of CRISPR towards accurate and efficient mammal genome engineering[J]. BMB reports, 2019, 52(8): 475.
[6]. Xiang X, Corsi G I, Anthon C, et al. Enhancing CRISPR-Cas9 gRNA efficiency prediction by data integration and deep learning[J]. Nature communications, 2021, 12(1): 3238.
[7]. DeFrancesco L. Church to de-extinct woolly mammoths[J]. Nat Biotechnol, 2021, 39(10): 1171.
[8]. Greely H. Of science, CRISPR-Cas9, and Asilomar[J]. Law and Biosciences Blog, 2015, 4.
[9]. Shapiro B. Mammoth 2.0: will genome engineering resurrect extinct species? [J]. Genome Biology, 2015, 16(1): 1-3.
[10]. Application, controversy and future of CRISPR gene editing technology [J]. Science Chinese,2018(10):78-80.
Cite this article
Shen,Y. (2023). CRISPR/Cas system: A powerful tool for de-extinction. Theoretical and Natural Science,20,221-225.
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The datasets used and/or analyzed during the current study will be available from the authors upon reasonable request.
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Volume title: Proceedings of the 3rd International Conference on Biological Engineering and Medical Science
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