References
[1]. Komor, A., Kim, Y., Packer, M. et al. “Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage.” Nature 533, 420-44 (2016).
[2]. Gaudelli, N., Komor, A., Rees, H. et al. “Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage.” Nature 551, 464–471 (2017).
[3]. Zuo, E., Sun, Y., Wei, W. et al. “Cytosine base editor generates substantial off-target single-nucleotide variants in mouse embryos.” Science 364(6437), 289-292 (2019).
[4]. Jin, S., Zong,Y., Gao,Q. et al. “Cytosine, but not adenine, base editors induce genome-wide off-target mutations in rice.” Science 364(6437), 292-295 (2019).
[5]. Chen, Y., Chen, YX., Ping, Y. “Application of base editing systems in the treatment of genetic diseases.” Journal of Pharmaceutical Sciences, 55(07):1562-1572 (2020).
[6]. Landrum, J., Lee, M., Benson, M. et al. “ClinVar: public archive of interpretations of clinically relevant variants.” Nucleic Acids Res 44(D1): D862-8 (2016).
[7]. Anzalone, A.V., Randolph, P.B., Davis, J.R. et al. “Search-and-replace genome editing without double-strand breaks or donor DNA.” Nature 576, 149–157 (2019).
[8]. Chung J., et al. “Molecular changes in appearance of a cancer cell among normal HEK293T cells.” Journal of Bioscience and Bioengineering 123, 3 (2017).
[9]. Doman, J.L., Raguram, A., Newby, G.A. et al. “Evaluation and minimization of Cas9-independent off-target DNA editing by cytosine base editors.” Nat Biotechnol 38, 620–628 (2020).
[10]. Zuo, E., Sun, Y., Yuan, T. et al. “A rationally engineered cytosine base editor retains high on-target activity while reducing both DNA and RNA off-target effects.” Nature Methods, 17(6), 600-604 (2020).
[11]. Zuo, E., Sun, Y., Wei, W. et al. “GOTI, a method to identify genome-wide off-target effects of genome editing in mouse embryos.” Nat Protoc 15, 3009–3029 (2020).
[12]. Jin, S., Lin, Q., Luo, Y. et al. “Genome-wide specificity of prime editors in plants.” Nat Biotechnol 39, 1292–1299 (2021).
[13]. Chen, P., Hussmann, J. et al. “Enhanced prime editing systems by manipulating cellular determinants of editing outcomes.” Cell. 184(22):5635-5652.e29 (2021).
[14]. Jiang, T., Zhang, XO., Weng, Z. et al. “Deletion and replacement of long genomic sequences using prime editing.” Nat Biotechnol 40, 227–234 (2022).
[15]. Choi, J., Chen, W., Suiter, C.C. et al. “Precise genomic deletions using paired prime editing.” Nat Biotechnol 40, 218–226 (2022).
[16]. Davis, J.R., Banskota, S., Levy, J.M. et al. “Efficient prime editing in mouse brain, liver and heart with dual AAVs.” Nat Biotechnol (2023).
Cite this article
Zhang,J. (2023). Research progress of high precision single base editing technology with a focus on prime editing. Theoretical and Natural Science,23,22-27.
Data availability
The datasets used and/or analyzed during the current study will be available from the authors upon reasonable request.
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References
[1]. Komor, A., Kim, Y., Packer, M. et al. “Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage.” Nature 533, 420-44 (2016).
[2]. Gaudelli, N., Komor, A., Rees, H. et al. “Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage.” Nature 551, 464–471 (2017).
[3]. Zuo, E., Sun, Y., Wei, W. et al. “Cytosine base editor generates substantial off-target single-nucleotide variants in mouse embryos.” Science 364(6437), 289-292 (2019).
[4]. Jin, S., Zong,Y., Gao,Q. et al. “Cytosine, but not adenine, base editors induce genome-wide off-target mutations in rice.” Science 364(6437), 292-295 (2019).
[5]. Chen, Y., Chen, YX., Ping, Y. “Application of base editing systems in the treatment of genetic diseases.” Journal of Pharmaceutical Sciences, 55(07):1562-1572 (2020).
[6]. Landrum, J., Lee, M., Benson, M. et al. “ClinVar: public archive of interpretations of clinically relevant variants.” Nucleic Acids Res 44(D1): D862-8 (2016).
[7]. Anzalone, A.V., Randolph, P.B., Davis, J.R. et al. “Search-and-replace genome editing without double-strand breaks or donor DNA.” Nature 576, 149–157 (2019).
[8]. Chung J., et al. “Molecular changes in appearance of a cancer cell among normal HEK293T cells.” Journal of Bioscience and Bioengineering 123, 3 (2017).
[9]. Doman, J.L., Raguram, A., Newby, G.A. et al. “Evaluation and minimization of Cas9-independent off-target DNA editing by cytosine base editors.” Nat Biotechnol 38, 620–628 (2020).
[10]. Zuo, E., Sun, Y., Yuan, T. et al. “A rationally engineered cytosine base editor retains high on-target activity while reducing both DNA and RNA off-target effects.” Nature Methods, 17(6), 600-604 (2020).
[11]. Zuo, E., Sun, Y., Wei, W. et al. “GOTI, a method to identify genome-wide off-target effects of genome editing in mouse embryos.” Nat Protoc 15, 3009–3029 (2020).
[12]. Jin, S., Lin, Q., Luo, Y. et al. “Genome-wide specificity of prime editors in plants.” Nat Biotechnol 39, 1292–1299 (2021).
[13]. Chen, P., Hussmann, J. et al. “Enhanced prime editing systems by manipulating cellular determinants of editing outcomes.” Cell. 184(22):5635-5652.e29 (2021).
[14]. Jiang, T., Zhang, XO., Weng, Z. et al. “Deletion and replacement of long genomic sequences using prime editing.” Nat Biotechnol 40, 227–234 (2022).
[15]. Choi, J., Chen, W., Suiter, C.C. et al. “Precise genomic deletions using paired prime editing.” Nat Biotechnol 40, 218–226 (2022).
[16]. Davis, J.R., Banskota, S., Levy, J.M. et al. “Efficient prime editing in mouse brain, liver and heart with dual AAVs.” Nat Biotechnol (2023).