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Investigating the prevalence and function of the mecA gene and PBP2a protein among Staphylococcus and Mammaliicoccus species
- 1 The Experimental High School Attached to Beijing Normal University
* Author to whom correspondence should be addressed.
Abstract
The mecA gene, an acquired gene encoding an additional penicillin-binding protein (PBP2a) with low affinity to nearly all β-lactams, is associated with the epidemiologically most important mechanism of antibiotic resistance in Staphylococcus aureus. However, apart from S. aureus, the mecA gene and functional PBP2a protein have also been discovered in other staphylococcal or mammalicoccal species. This research uses the Basic Local Alignment Search Tool (BLAST) to gather mecA gene and PBP2a amino acid sequences from multiple bacterial species and analyse the topology, structure, and function of the aligned PBP2a proteins. BLAST analysis indicated that the mecA gene and PBP2a protein sequences are present in several staphylococcal and mammaliicoccal species, and both the structure and function of the PBP2a protein are highly conserved among the species. This research indicates that the mecA gene and PBP2a protein are present and functional in a wide range of staphylococcal and mammaliicoccal species and highlights the high conserveness of the PBP2a protein among those species.
Keywords
Staphylococcus, drug resistance, bioinformatics
[1]. Gnanamani A, Hariharan P, Paul-Satyaseela M. Staphylococcus aureus: Overview of Bacteriology, Clinical Diseases, Epidemiology, Antibiotic Resistance and Therapeutic Approach. 2017. Frontiers in S. aureus, InTech; https://doi.org/10.5772/67338
[2]. Turner NA, Sharma-Kuinkel BK, Maskarinec SA, Eichenberger EM, Shah PP, Carugati M, et al. Methicillin-resistant Staphylococcus aureus: an overview of basic and clinical research. 2019 Nat. Rev. Microbiol. 17 203–18. https://doi.org/10.1038/s41579-018-0147-4
[3]. Fowler VG, Miro JM, Hoen B, Cabell CH, Abrutyn E, Rubinstein E, et al. Staphylococcus aureus Endocarditis A Consequence of Medical Progress. 2005 JAMA 293(24) 3012–3021. https://doi.org/10.1001/jama.293.24.3012
[4]. Tong SYC, Davis JS, Eichenberger E, Holland TL, Fowler VG. Staphylococcus aureus infections: Epidemiology, pathophysiology, clinical manifestations, and management. 2015 Clin. Microbiol Rev. 28 603–61. https://doi.org/10.1128/CMR.00134-14
[5]. Hassoun A, Linden PK, Friedman B. Incidence, prevalence, and management of MRSA bacteremia across patient populations-a review of recent developments in MRSA management and treatment. 2017 Crit. Care 21 211. https://doi.org/10.1186/s13054-017-1801-3
[6]. Pastagia M, Kleinman LC, de la Cruz EGL, Jenkins SG. Predicting risk for death from MRSA bacteremia. 2012 Emerg. Infect. Dis. 18 1072–80. https://doi.org/10.3201/eid1807.101371
[7]. American Thoracic Society. Guidelines for the management of adults with hospital-acquired, ventilator-associated, and healthcare-associated pneumonia. 2005 Am. J. Respir. Crit. Care Med 171 388–416. https://doi.org/10.1164/rccm.200405-644ST
[8]. Matuszewska M, Murray GGR, Harrison EM, Holmes MA, Weinert LA. The Evolutionary Genomics of Host Specificity in Staphylococcus aureus. 2020 Trends Microbiol. 28 465–77. https://doi.org/10.1016/j.tim.2019.12.007
[9]. Lee J H. Occurrence of methicillin-resistant Staphylococcus aureus strains from cattle and chicken, and analyses of their mecA, mecR1 and mecI genes. 2006 Vet. Microbiol. 114 155–9. https://doi.org/10.1016/j.vetmic.2005.10.024
[10]. Lowy FD. Antimicrobial resistance: the example of Staphylococcus aureus. 2003 J. of Clinical Investigation 111 1265–73. https://doi.org/10.1172/jci200318535
[11]. Jensen SO, Lyon BR. Genetics of antimicrobial resistance in Staphylococcus aureus. 2009 Future Microbiol. 4 565–82. https://doi.org/10.2217/fmb.09.30
[12]. Foster TJ. Antibiotic resistance in Staphylococcus aureus. Current status and future prospects. 2017 FEMS Microbiol. Rev. 41 430–49. https://doi.org/10.1093/femsre/fux007
[13]. Enright MC, Robinson DA, Randle G, Feil EJ, Grundmann H, Spratt BG. The evolutionary history of methicillin-resistant Staphylococcus aureus (MRSA). 2002 Proc. Natl. Acad. Sci. USA 99.; 7687–7692
[14]. Katayama Y, Ito T, Hiramatsu K. A New Class of Genetic Element, Staphylococcus Cassette Chromosome mec, Encodes Methicillin Resistance in Staphylococcus aureus. 2000 Antimicrobial agents and chemotherapy 44 1549–1555
[15]. Ito T, Kuwahara K, Hiramatsu K. Staphylococcal Cassette Chromosome mec (SCCmec) Analysis of MRSA. 2013 Methods of Mol. Bio. 1085 131–148
[16]. Hartman BJ, Tomasz A, Sabath L. Low-Affinity Penicillin-Binding Protein Associated with r-Lactam Resistance in Staphylococcus aureus methicillin-resistant strain Col was supplied. 1984 J. Bacteriol. 158.
[17]. Gardete S, Tomasz A. Mechanisms of vancomycin resistance in Staphylococcus aureus. 2014 J. Clinical Investigation 124 2836–40. https://doi.org/10.1172/JCI68834.
[18]. Murray CJ, Ikuta KS, Sharara F, Swetschinski L, Robles Aguilar G, Gray A, et al. Global burden of bacterial antimicrobial resistance in 2019: a systematic analysis. 2022 The Lancet 399 629–55. https://doi.org/10.1016/S0140-6736(21)02724-0
[19]. Williamson K, Bisaga A, Paquette K, Lovell E. The prevalence of methicillin-resistant Staphylococcus aureus colonization in emergency department fast track patients. 2013 World J. Emerg. Med. 4 278. https://doi.org/10.5847/wjem.j.issn.1920-8642.2013.04.006
[20]. Najar-Peerayeh S, Moghadas AJ, Behmanesh M. Antibiotic susceptibility and mecA frequency in staphylococcus epidermidis, isolated from intensive care unit patients. 2014 Jundishapur J. Microbiol. 7(8) https://doi.org/10.5812/jjm.11188
[21]. Gagetti P, Errecalde L, Wattam AR, De Belder D, Ojeda Saavedra M, Corso A, et al. Characterization of the First mecA-Positive Multidrug-Resistant Staphylococcus pseudintermedius Isolated from an Argentinian Patient. 2020 Microbial Drug Resistance 26 717–21. https://doi.org/10.1089/mdr.2019.0308.
[22]. Fuda C, Suvorov M, Shi Q, Hesek D, Lee M, Mobashery S. Shared functional attributes between the mecA gene product of Staphylococcus sciuri and penicillin-binding protein 2a of methicillin-resistant Staphylococcus aureus. 2007 Biochemistry 46 8050–7. https://doi.org/10.1021/bi7004587.
[23]. Tsubakishita S, Kuwahara-Arai K, Sasaki T, Hiramatsu K. Origin and molecular evolution of the determinant of methicillin resistance in staphylococci. 2010 Antimicrob. Agents Chemotherapy 54 4352–9. https://doi.org/10.1128/AAC.00356-10.
[24]. Gill SR, Fouts DE, Archer GL, Mongodin EF, DeBoy RT, Ravel J, et al. Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain. 2005 J. Bacteriol. 187 2426–38. https://doi.org/10.1128/JB.187.7.2426-2438.2005.
[25]. Bateman A, Martin MJ, Orchard S, Magrane M, Agivetova R, Ahmad S, et al. UniProt: the universal protein knowledgebase in 2021 2021 Nucleic Acids Res. 49 480–9. https://doi.org/10.1093/nar/gkaa1100.
Cite this article
Liu,Y. (2024). Investigating the prevalence and function of the mecA gene and PBP2a protein among Staphylococcus and Mammaliicoccus species. Theoretical and Natural Science,35,119-125.
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The datasets used and/or analyzed during the current study will be available from the authors upon reasonable request.
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