
Identification of cell types in the mouse spinal cord based on single cell RNA sequencing
- 1 School of Global Public Health, New York University, NY, 10003
* Author to whom correspondence should be addressed.
Abstract
The spinal cord is critical part of the central nervous system, harboring heterogeneous cell types. However, the genetic markers for various types of cells in the spinal cord remains to be elucidated. In this study, we analyzed the single cell RNA seq data to investigate the potential genetic markers for the heterogeneous cell types in the mouse spinal cord. Further, combined with immunohistology dataset, we determined the spatial distribution of these cell types in the mouse spinal cord. In this study, the identification of various types of cells in the mouse spinal cord would pave the way for future functional study which may underlie motor or sensory dysfunction
Keywords
mouse spinal cord, single cell RNA seq, cell types
[1]. Anupama Sathyamurthy, Anupama Sathyamurthy, Kory R. Johnson, Kaya J.E. Matson, Courtney I. Dobrott, Li Li, Li Li, et al. 2018. “Massively Parallel Single Nucleus Transcriptional Profiling Defines Spinal Cord Neurons and Their Activity during Behavior.” Cell Reports. https://doi.org/10.1016/j.celrep.2018.02.003.
[2]. Barry, Devin M., Xueting Liu, Xue-Ting Liu, Xue Ting Liu, Ben-Long Liu, Xian-Yu Liu, Xian-Yu Liu, et al. 2020. “Exploration of Sensory and Spinal Neurons Expressing Gastrin-Releasing Peptide in Itch and Pain Related Behaviors.” Nature Communications 11 (1): 1397. https://doi.org/10.1038/s41467-020-15230-y.
[3]. Bourane, Steeve, Katja S. Grossmann, Olivier Britz, Antoine Dalet, Marta Garcia Del Barrio, Floor J. Stam, Lidia Garcia-Campmany, Stephanie C. Koch, Stephanie C. Koch, and Martyn Goulding. 2015. “Identification of a Spinal Circuit for Light Touch and Fine Motor Control.” Cell 160 (3): 503–15. https://doi.org/10.1016/j.cell.2015.01.011.
[4]. Courtney I. Dobrott, Anupama Sathyamurthy, Anupama Sathyamurthy, Ariel J. Levine, and Ariel J. Levine. 2019. “Decoding Cell Type Diversity within the Spinal Cord.” Current Opinion in Physiology. https://doi.org/10.1016/j.cophys.2018.11.006.
[5]. Koch, Stephanie C., David Acton, and Martyn Goulding. 2018. “Spinal Circuits for Touch, Pain, and Itch.” Annual Review of Physiology 80 (1): 189–217. https://doi.org/10.1146/annurev-physiol-022516-034303.
[6]. Martin Häring, Amit Zeisel, Hannah Hochgerner, Puneet Rinwa, Puneet Rinwa, Jon E. T. Jakobsson, Peter Lönnerberg, et al. 2018. “Neuronal Atlas of the Dorsal Horn Defines Its Architecture and Links Sensory Input to Transcriptional Cell Types.” Nature Neuroscience. https://doi.org/10.1038/s41593-018-0141-1.
[7]. Osseward, Peter J, and Samuel L Pfaff. 2019. “Cell Type and Circuit Modules in the Spinal Cord.” Current Opinion in Neurobiology 56 (June): 175–84. https://doi.org/10.1016/j.conb.2019.03.003.
[8]. Rowitch, David H., and Arnold R. Kriegstein. 2010. “Developmental Genetics of Vertebrate Glial-Cell Specification.” Nature 468 (7321): 214–22. https://doi.org/10.1038/nature09611.
[9]. Sathyamurthy, Anupama, Anupama Sathyamurthy, Kory R. Johnson, Kaya J.E. Matson, Courtney I. Dobrott, Li Li, Li Li, et al. 2018. “Massively Parallel Single Nucleus Transcriptional Profiling Defines Spinal Cord Neurons and Their Activity during Behavior.” Cell Reports 22 (8): 2216–25. https://doi.org/10.1016/j.celrep.2018.02.003.
[10]. Silva, Nuno A., Nuno Sousa, Rui L. Reis, and António J. Salgado. 2014. “From Basics to Clinical: A Comprehensive Review on Spinal Cord Injury.” Progress in Neurobiology 114 (March): 25–57. https://doi.org/10.1016/j.pneurobio.2013.11.002.
[11]. Steeve Bourane, Katja S. Grossmann, Olivier Britz, Antoine Dalet, Marta Garcia Del Barrio, Floor J. Stam, Lidia Garcia-Campmany, Stephanie C. Koch, Stephanie C. Koch, and Martyn Goulding. 2015. “Identification of a Spinal Circuit for Light Touch and Fine Motor Control.” Cell. https://doi.org/10.1016/j.cell.2015.01.011.
Cite this article
Feng,X. (2025). Identification of cell types in the mouse spinal cord based on single cell RNA sequencing. Theoretical and Natural Science,73,159-171.
Data availability
The datasets used and/or analyzed during the current study will be available from the authors upon reasonable request.
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Volume title: Proceedings of the 4th International Conference on Biological Engineering and Medical Science
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